The effect of blocking integrins and their ligands.

<p>Two hours after the bead cluster had been inserted into the subcutaneous dorsal skin of C5BL6 mice, various blocking antibodies or isotype controls were injected intravenously. 3a: recruitment of neutrophils to the capillaries over ten minutes after treatment with either 30 µg anti-CD18 antibodies or isotype control antibodies. N = 3 independent experiments for mice given anti-CD18 antibodies, and 4 independent experiments for mice given isotype control antibodies. 3b: recruitment of neutrophils to the capillaries over ten minutes after treatment with either 30 µg anti-Mac 1 antibodies or isotype control antibodies. N = 5 independent experiments for Mac-1 and for isotype control experiments. 3c: recruitment of neutrophils to the capillaries over ten minutes after treatment with either 30 µg anti-LFA1 antibodies or isotype control antibodies. N = 5 independent experiments for mice treated with LFA-1 blocking antibodies and 4 independent experiments for isotype controls. 3d: recruitment of neutrophils to the capillaries over ten minutes after treatment with either CD18 and anti-α₄ antibodies, CD18 antibodies alone, anti-α₄ antibodies, or isotype controls. N = 3 independent experiments for all conditions. 3e: recruitment of neutrophils to the capillaries over ten minutes after treatment with 100 µg anti-ICAM-1 antibodies, anti-VCAM-1 antibodies, both antibody treatments or isotype control antibodies. N = 4 independent experiments for all condition states. Each N is an average of 4 or more 10 minute videos at 10× FOV from a single mouse. ** <i>p</i><0.01.