Tph1 gene ablation alter mammary epithelial cell morphology and cell proliferation on d 10 of lactation.

<p>Wild-type (WT) mouse dams were given saline and Tryptophan hydroxylase knock-out dams given either saline (<i>Tph1^−/−</i>) or daily injections of exogenous 5-HTP (100 mg/kg; <i>Tph1^−/−</i> (5-HTP)), from pregnancy d20 to lactation d10. (A) Mammary gland Hematoxylin and Eosin (H&E) staining and quantification of (B) alveolar diameter and (C) number. (D) Immunofluorescence staining with Ki-67 proliferation marker (red) combined with E-cadherin (green) and DAPI (blue). (E) Quantification of Ki-67 positive cells. All values are the mean ± SEM (n = 4). Groups with different letters are significantly different (<i>P</i><0.05).