Competition assay to assess FGF2 dimerization (C-N orientation) using the AlphaScreen^™ technology.

<p>A. Competition assay of FGF2 dimerization with heparin-like substances. The interaction between 50 nM FGF2-N-GST and 50 nM FGF2-C-His <i>was</i> competed in the presence of increasing concentrations of heparin. Tag less FGF2 (B) and PF4 (C) compete for FGF2 dimerization of 50 nM FGF2-N-GST and 50 nM FGF2-C-His. D. Anionic citrate inhibits heparin-dependent and independent FGF2 dimerization. (A through D) AlphaScreen^™ signal is expressed as percentages in comparison of control; AlphaScreen^™ signal corresponding to FGF2 dimer without competitors is set as 100%. Results are representative of at least three independent experiments. Results are mean values ± SEM (n = 3).