Carnosol inhibits MDA-MB-231 colony growth.

<p>Carnosol induced arrest of MDA-MB-231 colony growth. MDA-MB-231 colonies were first allowed to form in normal media for 2 weeks as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2" target="_blank">Material and Methods</a>. Formed colonies were then treated with DMSO or 25 µM carnosol and allowed to grow for one more week before staining. Inhibition of colony growth was assessed by measuring the size of the colonies obtained in DMSO and carnosol-treated plate. Data were compared with those obtained for the 2 weeks colonies. The size of the colonies were measured, counted using a microscope (10×) and the colony size was categorized as Large (≥200 µM), or small (50–200 µM). Student's t test was performed to determine the significance (*<i>p</i><0.05, **<i>p</i><0.01 and ***<i>p</i><0.005).