β-catenin forms punctate nuclear lesions in response to PKA activation in primary cultures.

<p>Primary osteoblasts from wild type (WT) bones or from bone tumors arising in <i>Prkar1a^+/−</i> mice were studied by immunofluorescence for β-catenin (green). For reference, cell nuclei were stained with DAPI. The left column shows β-catenin, only, whereas the right column shows merger of the β-catenin and DAPI stains. Top) WT osteoblasts. Middle) Tumor osteoblasts. Bottom) WT osteoblasts treated with forskolin (FSK). Note the punctate nuclear localization of β-catenin observed in Tumor cells or WT cells treated with FSK. Magnification: 400x.