Binding of YB2/0- and NS0-produced Fog-1 G1 antibodies to human FcγR.

<p><b>A</b> Binding of monomeric IgG was measured for FcγRI using the B2KA cell line and flow cytometry. Fog-1 IgG2 antibody, produced in the YB2/0 cell line, was used as the non-binding control antibody for this receptor. <b>B–F</b> Binding of pre-complexed IgG was measured using CHO cell lines expressing the low affinity receptors which were FcγRIIa, allotypes 131R (B) and 131H (C), FcγRIIb (D) and FcγRIIIb, allotypes NA1 (E) and NA2 (F). The level of background binding is given by the negative control antibody, IgA,κ. Graphs show mean fluorescence of ≥12 000 cells at each antibody concentration and are typical of the results obtained in at least three experiments with each receptor.