More pronounced reactivation was induced by PAF in neutrophils desensitized by the pepducin F2Pal₁₀.

<p><b>A.</b> Naïve neutrophils were activated with different concentrations of WKYMVM (10–40 nM; black bars) or F2Pal₁₀ (100–500 nM; white bars) followed by a second stimulation with PAF (100 nM final concentration) and the release of superoxide anions was recorded continuously. The results are given as peak values of superoxide production from three separate experiments for the first stimulation with FPR2 agonists (mean+SEM, n = 3). <b>B.</b> Neutrophils desensitized with different concentrations of WKYMVM (10–100 nM; WKYMVM_des., black bars) or F2Pal₁₀ (10–500 nM; F2Pal_10des., white bars) were reactivated by a second stimulation with PAF (100 nM final concentration), after which the superoxide production (peak values) was recorded. The results are given as fold increase (peak values) of the PAF-induced response from FPR2-desensitized cells compared to the PAF response from naïve cells (mean+SEM; n = 3). The naïve PAF response (fold increase = 1) is indicated as a horizontal broken line.