The effect of recombinant HMGB1 on NF-ĸB p65 subunit expression and NF-ĸB-DNA binding.

<p>(A) Exposure to recombinant HMGB1 (500 ng/ml) in control media for 2 hours induced nuclear NF-ĸB p65 subunit expression and, (B) NF-ĸB-DNA binding was also induced with exposure to recombinant HMGB1 (500 ng/ml) in HMEC-1 cells. Normalized results are expressed as mean ± SEM, n = 5. *P<0.05 versus HMEC-1 cells cultured in the absence of recombinant HMGB1. **P<0.01 versus HMEC-1 cells cultured in the absence of recombinant HMGB1.