Overexpression of SRF by a SRF plasmid enhances HPMC EMT <i>in vitro</i>.

<p>(a) Western blot analysis of SRF in HPMCs transfected with a SRF plasmid or control plasmid. (b) Fluorescence microscopy showed the location and expression of the EMT markers E-cadherin, α-SMA and SRF in HPMCs transfected with a SRF plasmid or control plasmid. Magnification is 100×. (c) Real-time PCR showing mRNA of SRF, E-cadherin and α-SMA in HPMCs transfected with a SRF plasmid or control plasmid (*P<0.05 vs. control).