Sequence and phylogenetic analysis of ArPHB1.

<p>A, Schematic view of PHB1 architecture. B, Amino acid sequence alignment of ArPHB1 with PHB1 from other species. The sequences used in this alignment and their Genbank/EMBL/DDBJ or SWISS-PROT database accession numbers are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109152#pone.0109152.s006" target="_blank">Table S2</a>. Gaps inserted to maximize alignment are denoted by hyphens. The amino acid position numbers are shown to the right of the sequences. The phosphorylation sites against which the antibody was generated is marked by P. Conserved domains are marked by lines below sequences (the N-terminal hydrophobic domain by yellow, the PHB domain by black, the coiled-coil domain by blue, and the nuclear export sequence by red). The alignment was performed by MEGA5 using the ClustalW method. C, A phylogenetic tree of the amino acid sequences of prohibitins. The sequences used in this analysis and their GenBank/EMBL/DDBJ or SWISS-PROT database accession numbers are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109152#pone.0109152.s005" target="_blank">Table S1</a>. The phylogenetic tree was constructed using the Neighbor-joining method. Bootstrap percentage values for 1000 replicate analysis are shown at branching points. The bar at the bottom shows the branch length, and it corresponds to the mean number of differences (0.05) per residue along each branch.