Luteolin-induced cell cycle arrest and nuclear envelope disassembly defects.

<p>(A), Cell cycle analysis was carried out by flow cytometry. HeLa cells transfected with GFP or GFP-VRK1 were treated with luteolin for 24 hours, and 10,000 cells were gated for analysis. Quantitative data are below the histogram plots. (B) and (C), HeLa cells treated with vehicle or luteolin were stained with lamin B antibody to visualize nuclear envelope and with Hoechst 33342 to visualized DNA. Alexa 488 dye-conjugated antibody was used as secondary antibody. The slides were visualized by fluorescence microscopy (B), or confocal laser scanning microscopy (C). (D), Fluorescent staining of VRK1, BAF, and DNA for analysis of the phosphorylation-mediated re-localization of BAF. Cells co-transfected with GFP or GFP-BAF with RFP or RFP-VRK1 were treated with DMSO or 10 µM luteolin for 24 hours.