Direct interaction of luteolin with VRK1.

<p>(A), Pull-down assay using luteolin-conjugated sepharose 4B beads or control sepharose 4B beads with recombinant VRK1 protein. Purified GST and GST-VRK1 proteins are incubated with indicated beads, and then pull down assay was performed. Each proteins were detected by immunoblotting with GST antibody. (B), Pull-down assay using luteolin conjugated sepharose 4B beads with SH-SY5Y cell lysate. Cell lysate are incubated with indicated beads, and then pull-down was performed. The proteins were detected by immunoblotting with VRK1 antibody. (C), SPR detection for the interaction of luteolin with VRK1. The data were obtained by kinetic titration method with sequentially injection of analytes without regeneration steps. Data for eupatilin and wogonin were obtained by classical methods.