Proteins found in different amounts in the culture supernatants of the wild type and ΔsecDF mutant (P-value <0.05).

1<p>according to prediction of PSORTb algorithm (version 3.0.2; [26]): EC extracellular, C cytoplasmic, U unknown, M membrane; references for experimentally defined locations are given for proteins with predicted unknown localization.</p>2<p><b>N</b>ormalized <b>S</b>pectral <b>A</b>bundance <b>F</b>actor, mean average of three biological replicates; the NSAF normalizes across samples and takes protein sizes into account; values range between 0 and 1, increasing values indicate higher abundance [27], stdev standard deviation of the means of three biological replicates; probability ranges associated with Students t-test (Scaffold 4.0.5).</p>3<p>ND not detected (NSAF 0 in at least two biological replicates and <0.005).</p><p>*due to high sequence similarity all peptide hits for “flagellin” (Q81FD3, Q81FD4, Q81FD5) were combined.</p><p>Proteins found in different amounts in the culture supernatants of the wild type and ΔsecDF mutant (P-value <0.05).