<i>In vitro,</i> CB1a can disrupt lung cancer cells from adhering together to form tumor-like spheroids.

<p>5×10² NCI-H460 lung cancer cells were introduced to each cell plate (hanging drop method, refer <i>Methods</i>). In the left column (<i>A</i>), plates were “pre-treated” with CB1a before the introduction of cells. In the right column (<i>B</i>), plates were “post-treated” with CB1a after the introduction of cells. The concentration of CB1a applied is increased as we go vertically down the columns: 5, 15, 30, 45 and 60 µM for panels a, b, c, d and e respectively. With post-treatment, spheroids are still observed. With pre-treatment: the greater the CB1a concentration used, the smaller the spheroid; until - with higher CB1a concentrations - no spheroids form. High CB1a concentrations can block cancer cell adhesions.