Time-depended influence of bortezomib on TRAIL or DR5-B mediated cell death in HCT116 p53^+/+ and HCT116 p53^−/− cells.

<p>(A) Viability of the cells treated with different concentrations of TRAIL or DR5-B during 8, 16, 20 and 24 h of incubation. (B) Viability of the cells treated with 1 nM bortezomib and different concentrations of TRAIL or DR5-B during 8, 16, 20 and 24 h of incubation. (C) Cells were incubated with different concentrations of bortezomib and cell death was measured after 24 h of incubation. (D) Calculation of effective concentrations of TRAIL variants after 24 h treatment of cells with 1 nM bortezomib. Values are mean ± SD of at least three independent experiments.