Balb/c response against 4 H-2Kd restricted peptides.

<p>4 mice were immunized with polytope construct three times with one week interval and sacrificed 10 days after the last booster. Splenocytes from individual mice were <i>in-vitro</i> re-stimulated by representative peptides (Kd1-4) of Balb/c and specific IFN-γ production was evaluated by <i>ex-vivo</i> ELISPOT assay. A. Representative of two experiments. Columns, mean of spots from duplicate wells for each mice from one representative experiment; bars, SD. Stimulations resulting in spots two times the negative control (unstimulated cells) and more than 10 were considered positive (stars). B. Statistical analysis of consolidated data from 4 mice against each individual peptide. The response against all 4 peptides appeared statistically significant compared to un-stimulated control cells (<i>p</i><0.05) with an exception for Kd4 which was subdominant in comparison to the rest. Horizontal lines represent the mean value. SFC: Spot Forming Cells.