<p>A549 cells expressing native FL IFITM3, Δ21 IFITM3, or Y20A IFITM3 were fixed, permeabilized, and labeled with anti-LAMP2 (green) and anti-IFITM2/3 (red) and DAPI (blue) antibodies. DAPI was used as nuclear counterstain. Cells were imaged by confocal microscopy. Each panel represents at least two sets of experiments with similar results.</p
<p>A549 (A), 293T (C), or Vero E6 (E) cells transduced with vector alone or to express FL IFITM3, Δ2...
<p>PBMC from LTBI+ donors were stimulated with PPD or left unstimulated, stained with antibodies aga...
<p>A20 B cells stably expressing M2 or M2Y were pulsed overnight, or not, with different concentrati...
<p>A549 cells expressing the indicated c-myc- (A) or flag-tagged (B) IFITM3 isoforms were fixed, per...
<p>Immunofluorescence of A549 cells expressing HA-tagged IFITM1, IFITM2 and IFITM3 (or control A549 ...
<p>(A) HEK293 cells were transfected with plasmid DNA expressing wild type IFITM1 and its mutants Δ(...
<p>C-terminal domain HA-tagged IFITM1, IFITM2, IFITM3 and control A549 cell lines were stained intac...
<p>Live A549-IFITM-HA cells were incubated with 5 µg/ml anti-HA at 37°C to allow endocytosis of boun...
<p>Permeabilised IFITM2-HA (A) and IFITM3-HA (B) expressing cells were stained with antibodies again...
<p>BMDM were prepared from B6.C3H-<i>sst1</i> mice. Where indicated (panel a, c and d), cells were p...
<p>(A–D) and (H–J). Conventional IF microscopy using anti-fiber monoclonal antibody TB5 and secondar...
<p>Cells were transfected with BAT3 splice variants and stained after 24 hours with a monoclonal V5 ...
<p>Two commercially available antibodies targeting either the IFITM1-NTD or the IFITM3-NTD were scre...
<p>(A) Sub-cellular distributions of cholesterol and IFITM3 in A549-Vector and A549-IFITM3 cells. Ch...
<p>(A). Western blot of Vero cell lines expressing IFITM1, 2 and 3. (B). Graphics depict mean±SD of ...
<p>A549 (A), 293T (C), or Vero E6 (E) cells transduced with vector alone or to express FL IFITM3, Δ2...
<p>PBMC from LTBI+ donors were stimulated with PPD or left unstimulated, stained with antibodies aga...
<p>A20 B cells stably expressing M2 or M2Y were pulsed overnight, or not, with different concentrati...
<p>A549 cells expressing the indicated c-myc- (A) or flag-tagged (B) IFITM3 isoforms were fixed, per...
<p>Immunofluorescence of A549 cells expressing HA-tagged IFITM1, IFITM2 and IFITM3 (or control A549 ...
<p>(A) HEK293 cells were transfected with plasmid DNA expressing wild type IFITM1 and its mutants Δ(...
<p>C-terminal domain HA-tagged IFITM1, IFITM2, IFITM3 and control A549 cell lines were stained intac...
<p>Live A549-IFITM-HA cells were incubated with 5 µg/ml anti-HA at 37°C to allow endocytosis of boun...
<p>Permeabilised IFITM2-HA (A) and IFITM3-HA (B) expressing cells were stained with antibodies again...
<p>BMDM were prepared from B6.C3H-<i>sst1</i> mice. Where indicated (panel a, c and d), cells were p...
<p>(A–D) and (H–J). Conventional IF microscopy using anti-fiber monoclonal antibody TB5 and secondar...
<p>Cells were transfected with BAT3 splice variants and stained after 24 hours with a monoclonal V5 ...
<p>Two commercially available antibodies targeting either the IFITM1-NTD or the IFITM3-NTD were scre...
<p>(A) Sub-cellular distributions of cholesterol and IFITM3 in A549-Vector and A549-IFITM3 cells. Ch...
<p>(A). Western blot of Vero cell lines expressing IFITM1, 2 and 3. (B). Graphics depict mean±SD of ...
<p>A549 (A), 293T (C), or Vero E6 (E) cells transduced with vector alone or to express FL IFITM3, Δ2...
<p>PBMC from LTBI+ donors were stimulated with PPD or left unstimulated, stained with antibodies aga...
<p>A20 B cells stably expressing M2 or M2Y were pulsed overnight, or not, with different concentrati...