Expression of Onecut1 and Onecut2 in the developing murine retina.

<p>The expression of Onecut transcription factors was analyzed throughout retinal development (A). A heatmap indicating the genes (rows) expressed in isolated single retinal progenitor cells (columns) at various stages of development, from embryonic day (E)12.5 to E16.5. Increased expression of a gene in a given cell is indicated in shades of red, while the absence of expression is indicated with a black square. Expression patterns of Onecut1 (B) and Onecut2 (C) mRNAs were determined via in situ hybridization at various stages of retinal development and in the adult retina. Arrowheads indicate expression in the ganglion cell layer. Adult scale bars represent 100 µm; all others 200 µm. (D,E) Dissociated cell in situ hybridization was performed at E14.5 using a probe targeting either Onecut1 or Onecut2 and Math5. Arrowheads indicate overlapping Math5 and Onecut family member expression. (F) Quantification of dissociated retinal cells expressing Math5, Onecut1, and Onecut2.