Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells).

<p>A. Expression of CD56 and CD3 on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 Dynabeads, generating cell populations comprising >95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).