MHCII expression on recipients is required for liver inflammation mediated by H2M−/− CD4 T cells.

<p>(A) Liver pathogenesis in Rag−/− and H2M−/− Rag−/− recipients after H2M−/− T cell transfer. (B) Absolute numbers of donor CD4 T cells were analyzed 7 days post transfer. (C) IFNγ and IL-17A cytokine expression were determined by FACS analysis. (D and E) Lethally irradiated MHCII−/− Rag−/− mice were reconstituted with BM cells from Rag−/− (Rag−/− BM → MHCII−/− Rag−/−, WT → KO). FACS purified naive H2M−/− CD4 T cells were transferred into the reconstituted recipients after 6 weeks of BM reconstitution. Recipients were sacrificed 7 days post transfer. (D) Liver pathology and (E) absolute numbers of donor cells were assessed. All experiments were repeated twice and similar results were observed. Each symbol represents individually tested animals. *, p<0.05; **, p<0.01.