<p>(<b>A</b>) mRNA expression of osteoblast marker genes in 14-day osteogenic cultures of BMSCs in the presence of the Smoothened agonist. mRNA expression was analyzed by real-time RT-PCR. (<b>B</b>) ALP and von Kossa stainings in 14-day osteogenic cultures of BMSCs in the presence of the Smoothened agonist. (<b>C</b>) mRNA expression of osteoblast marker genes in 7-day osteogenic cultures of osteoblast precursors (OPs) isolated from neonatal calvarias, in the presence of the Smoothened agonist. (<b>D</b>) ALP and von Kossa staining in 7-day osteogenic cultures of OPs in the presence of the Smoothened agonist. (<b>E</b>) Rescue of the expression levels of osteoblast marker genes in <i>Gli1</i><sup>+/−</sup> OPs by the adenoviral overexpress...
Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesench...
<p>(<b>A</b>) Serum Pi and FGF23 levels in 20-week-old <i>Hyp</i> (male hemizygotes and female heter...
<p>(A) Lrp5 mRNA levels in primary calvarial osteoblasts were assessed by qPCR during 14 days of ost...
<p>(<b>A</b>) Formation of TRAP-positive multinucleated osteoclasts by the co-culture of BMSCs and B...
<p>Total RNA was isolated from MSC and cultured for 7 days. The levels of transcripts were determine...
(B) mRNA and protein levels of Runx2 and CHIP during the osteoblast differentiation were compared. R...
<p>Bone marrow cells were extracted from 3-month-old mice and cultured with ascorbic acid and β-glyc...
<p>(A–C) Induction of osteoblast differentiation by FoxO3aTM. Primary osteoblasts from calvariae of ...
<p>Osteoblasts were classically obtained after treatment of bone marrow cells with ß sodium-glycerop...
<p>(<b>A and B</b>) Formation of colony forming units-osteoblasts (CFU-OB) in ex-vivo cultures of bo...
<p>(A) Real-time RT-PCR analysis of <i>Bcl2</i>, <i>Runx2</i>, <i>Osterix</i>, <i>Col1a1</i>, <i>ost...
<p><b>A</b>) Upper panels: Alkaline phosphatase (ALP)- and von Kossa staining after 7 d and 21 d, re...
<p>(A) MTT assay. Primary osteoblasts from calvariae of 4 wild-type and 3 Bcl2<sup>−/−</sup> mice we...
<p>Real-time PCR was performed with total RNA isolated from the long bones of the one-week-old contr...
<p><b>A</b>. Experimental scheme: calvarial cells from newborn WT and Akt1 KO mice were incubated in...
Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesench...
<p>(<b>A</b>) Serum Pi and FGF23 levels in 20-week-old <i>Hyp</i> (male hemizygotes and female heter...
<p>(A) Lrp5 mRNA levels in primary calvarial osteoblasts were assessed by qPCR during 14 days of ost...
<p>(<b>A</b>) Formation of TRAP-positive multinucleated osteoclasts by the co-culture of BMSCs and B...
<p>Total RNA was isolated from MSC and cultured for 7 days. The levels of transcripts were determine...
(B) mRNA and protein levels of Runx2 and CHIP during the osteoblast differentiation were compared. R...
<p>Bone marrow cells were extracted from 3-month-old mice and cultured with ascorbic acid and β-glyc...
<p>(A–C) Induction of osteoblast differentiation by FoxO3aTM. Primary osteoblasts from calvariae of ...
<p>Osteoblasts were classically obtained after treatment of bone marrow cells with ß sodium-glycerop...
<p>(<b>A and B</b>) Formation of colony forming units-osteoblasts (CFU-OB) in ex-vivo cultures of bo...
<p>(A) Real-time RT-PCR analysis of <i>Bcl2</i>, <i>Runx2</i>, <i>Osterix</i>, <i>Col1a1</i>, <i>ost...
<p><b>A</b>) Upper panels: Alkaline phosphatase (ALP)- and von Kossa staining after 7 d and 21 d, re...
<p>(A) MTT assay. Primary osteoblasts from calvariae of 4 wild-type and 3 Bcl2<sup>−/−</sup> mice we...
<p>Real-time PCR was performed with total RNA isolated from the long bones of the one-week-old contr...
<p><b>A</b>. Experimental scheme: calvarial cells from newborn WT and Akt1 KO mice were incubated in...
Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesench...
<p>(<b>A</b>) Serum Pi and FGF23 levels in 20-week-old <i>Hyp</i> (male hemizygotes and female heter...
<p>(A) Lrp5 mRNA levels in primary calvarial osteoblasts were assessed by qPCR during 14 days of ost...