Histological findings of adult WT and <i>Gli1</i>^+/− mice.

<p>(<b>A</b>) von Kossa staining and toluidine blue staining of the distal femur sections of representative 8-week-old WT and <i>Gli1</i>^+/− male mice. Bar, 100 µm. (<b>B</b>) Histomorphometric analyses of bone volume and bone formation parameters in distal femurs from 8-week-old WT and <i>Gli1</i>^+/− male mice. OS/BS, osteoid surface per bone surface; MAR, mineral apposition rate; BFR, bone formation rate per bone surface; dLS/BS, double-labeled surface per bone surface; sLS/BS, single-labeled surface per bone surface. Data are means ± SDs of five male mice per genotype. *p<0.05 vs. WT. (<b>C</b>) TRAP staining of the distal femur sections of representative 8-week-old WT and <i>Gli1</i>^+/− male mice. Bar, 100 µm. (<b>D</b>) Histomorphometric analyses of bone resorption parameters in the distal femurs of 8-week-old WT and Gli1^+/− male mice. ES/BS, eroded surface per bone surface; N. Oc/B. Pm, number of osteoclasts per 100 mm of bone perimeter; Oc. S/BS, osteoclast surface per bone surface. In (<b>B</b>) and (<b>D</b>), data are means ± SDs of five mice per genotype. *p<0.05 vs. WT.