Over-expression of miR-199a represses growth and tumorigenicity <i>in vitro</i> and <i>in vivo</i>.

<p>(A) qRT-PCR analysis confirmed that miR-199a expression was significantly up-regulated in HepG2-199a cells compared with matched controls. U6 snRNA was used as the internal control. (B) The growth curves are plotted based on the MTT assay results. The value shown is the mean of three experiments. *Statistical significance. (C) Cell cycle distribution of HepG2, HepG2-CN, and HepG2-199a cells by flow cytometry. (D) The proliferation index (PI) of HepG2, HepG2-CN, and HepG2-199a cells, as determined by flow cytometry. The PI values that are shown are the mean of three repetitions and are expressed as the mean ± SD. (E) Colony numbers of HepG2-CN and HepG2-199a cells in soft agar. Each soft agar assay was performed in triplicate, and the results are expressed as the mean number of colonies ± SD. (F) Tumor size of HepG2-CN and HepG2-199a cells in nude mice. (G) MiR-199a expression in whole tumor tissue extracts by qRT-PCR analysis. (H) FZD7 expression in whole tumor tissue extracts by Western blot analysis.