Pole fragmentation is correlated with abnormal centriole splitting in CENP-W depleted cells.

<p>(<b>A</b>) Representative panel of GFP-CENP-W cells transfected with siCENP-W for 48 h, stained for pericentrin (for centrosome detection, green) and centrin-3 (for centriole detection, red). siCENP-W prometaphase cells exhibited multipolar spindles (pericentrin panel, red arrows indicate non-centriolar associated poles). (<b>B</b>) Graph represents mean percentage (+SEM) of mitotic cells with >2 pericentrin or >4 centrin foci (at least 100 cells were scored, in triplicate assays). (<b>C</b>) Graph represents mean percentage (+SEM) of mitotic cells with split centrioles. * p<0.01, *** p<0.0001 (one-way ANOVA followed by Dunnetts multiple comparison). Scale bar 5 µm.