Colony formation in stempro-34 and DMEM-FBS media with growth factors.

<p>Total testicular cells were cultured for 7 days in (A) stempro-34, (B) DMEM- 1%FBS, (C) DMEM- 5%FBS, and (D) DMEM- 1%FBS. Combinations of growth factors were added in each media. Arrowheads indicate the colonies formed in specific media conditions. Scale bars indicate 100 µm in all panels. GDNF, glial cell-derived neurotrophic factor; bFGF, basic fibroblast growth factor; LIF, leukemia inhibitory factor; EGF, epidermal growth factor. Magnification is ×100 in all panels.