Pretreatment with c-di-GMP reduces the bacterial load in the lungs of mice challenged with <i>B. pertussis</i>.

<p>Mice were intranasally treated with c-di-GMP or control (PBS) at 24 hr prior to challenge. Bacterial counts were determined at day 2, 4 and 6 post challenge. The results are expressed as the mean values ± the standard deviations per lung, as counted from individual lungs of 10 mice per group from three separate experiments. *, P<0.0001 in lung homogenates versus the control group.