Expression characteristics and function enrichment analysis of differentially expressed miRNAs/mRNAs.

<p>(<b>A, B</b>) The hierarchical cluster analysis of differentially expressed mRNAs and miRNAs in atherosclerosis (P) compared to controls (N). The fold changes of expression compared to controls were indicated by the color bar. (<b>C</b>) The intersection of potential target genes of differentially expressed miRNAs and differentially expressed mRNAs was merged with the differentially methylated genes. Then the functional enrichment analysis and activation simulation of them was performed by IPA. B-pvalue indicated Bonferroni's corrected p value. The solid line showed the significant threshold of functional enrichment (>−lg(0.05) = 1.3). The dashed line showed the significant threshold of activation and inhibition simulation (|z|≥2). (<b>D</b>) The clusters of gene expressions of some enriched functions. The fold changes of expression were indicated by the color bar at the bottom. (<b>E</b>) Eight genes that were significantly regulated at DNA methylation and mRNA expression levels were confirmed by qPCR and MSP. The results of the t test showed that their expressions/methylations were significantly changed in atherosclerosis, P<0.05, Number of samples were 20.