Motoneurons regenerate and form neuromuscular junctions.

<p>Longitudinal sections of gastrocnemius muscle showing end plates identified with α-bungarotoxin binding to acetylcholine receptors (red) and nerve terminal stained with neurofilament-200 (green). (A and B) Normal animal used as positive control; most end plates exhibited a “pretzel-like” shape with precise apposition of motor nerve terminals to the motor endplates, indicating that the endplate is perfectly innervated. (C, D and E) DMEM, TMT and SC groups showed neuromuscular junctions with disorganized and enlarged morphology, and exhibited poor and fragmented NF-200 staining. (F) The TMT + SC group displayed more “pretzel-like” junctions, similar to normal animal. Bar: (A) 50 µm (B, C, D, E and F) 25 µm.