<p>(A) Human proximal tubular epithelial cells were transfected with deptor shRNAs and siRNA against Hif1α. The transfected cells were incubated with TGFβ for 24 hours. The cell lysates were immunoblotted with collagen I (α2), deptor, Hif1α and actin antibodies as indicated. Quantifications of panel A is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109608#pone.0109608.s011" target="_blank">Fig. S11A</a>. (B) Collagen I (α2) promoter-driven luciferase reporter plasmid was co-transfected with deptor shRNAs (Deptor sh1 and Deptor sh2) and siRNA against Hif1α. The transfected cells were incubated with TGFβ for 24 hours. The cell lysates were assayed for luciferase activity as described in the <a href="http://w...
Background: By transdifferentiation, proximal tubular cells (PTC) have been considered as a source o...
Progressive renal disease is characterized by accumulation of extracellular matrix in the renal cort...
<p>(A–F) On day 0, A549 cells were seeded at 1×10<sup>5</sup> cells per 60 mm dish. On day 1, the ce...
<p>(A and B) Human proximal tubular epithelial cells were transfected with deptor shRNAs (deptor sh1...
<p>Human proximal tubular epithelial cells were transfected with collagen I (α2) promoter-driven luc...
<p>(A–C and E) Human proximal tubular epithelial cells were transfected with two independent shRNAs ...
<p>(A–D) TGFβ decreases deptor resulting in increased mTORC1 and mTORC2 activity. Human proximal tub...
<p>(A) Human proximal tubular epithelial cells were incubated with 2 ng/ml TGFβ for indicated period...
<p>Human proximal tubular epithelial cells were cotransfected with the Hif1α 5′UTR-fused Renilla luc...
<p>(A) The sequence showing the HRE (bases denoted in red and underlined), the start codon (in blue)...
Enhanced TGFβ activity contributes to the accumulation of matrix proteins including collagen I (α2) ...
<div><p>Enhanced TGFβ activity contributes to the accumulation of matrix proteins including collagen...
<p>Human RPTECs were cultivated with medium or 3 ng/ml TGF-β1 for 48 h, followed by incubation in fr...
<p>A. WF-A inhibits TGF-β1 induced increase in collagen polypeptides. HLFs were treated with DMSO (l...
<p><b>A) After 72 hours of stimulation with TGF-β<sub>1</sub>, the gene expression of collagen I and...
Background: By transdifferentiation, proximal tubular cells (PTC) have been considered as a source o...
Progressive renal disease is characterized by accumulation of extracellular matrix in the renal cort...
<p>(A–F) On day 0, A549 cells were seeded at 1×10<sup>5</sup> cells per 60 mm dish. On day 1, the ce...
<p>(A and B) Human proximal tubular epithelial cells were transfected with deptor shRNAs (deptor sh1...
<p>Human proximal tubular epithelial cells were transfected with collagen I (α2) promoter-driven luc...
<p>(A–C and E) Human proximal tubular epithelial cells were transfected with two independent shRNAs ...
<p>(A–D) TGFβ decreases deptor resulting in increased mTORC1 and mTORC2 activity. Human proximal tub...
<p>(A) Human proximal tubular epithelial cells were incubated with 2 ng/ml TGFβ for indicated period...
<p>Human proximal tubular epithelial cells were cotransfected with the Hif1α 5′UTR-fused Renilla luc...
<p>(A) The sequence showing the HRE (bases denoted in red and underlined), the start codon (in blue)...
Enhanced TGFβ activity contributes to the accumulation of matrix proteins including collagen I (α2) ...
<div><p>Enhanced TGFβ activity contributes to the accumulation of matrix proteins including collagen...
<p>Human RPTECs were cultivated with medium or 3 ng/ml TGF-β1 for 48 h, followed by incubation in fr...
<p>A. WF-A inhibits TGF-β1 induced increase in collagen polypeptides. HLFs were treated with DMSO (l...
<p><b>A) After 72 hours of stimulation with TGF-β<sub>1</sub>, the gene expression of collagen I and...
Background: By transdifferentiation, proximal tubular cells (PTC) have been considered as a source o...
Progressive renal disease is characterized by accumulation of extracellular matrix in the renal cort...
<p>(A–F) On day 0, A549 cells were seeded at 1×10<sup>5</sup> cells per 60 mm dish. On day 1, the ce...