Prediction of glycolytic metabolite pools during cultivation of adherent MDCK cells in DMEM with 2.5 mmol L⁻¹ extracellular glucose.

<p>Glucose 6-phosphate (<b>A</b>), fructose 6-phosphate (<b>B</b>), fructose 1,6-bisphosphate (<b>C</b>), 3-phosphoglyceric acid (<b>D</b>) and phosphoenolpyruvate (<b>E</b>) concentrations during MDCK cell cultivations in 6-well plates and DMEM medium with 2.5 mmol L⁻¹ extracellular glucose. Data (◊) and error bars represent mean and standard deviation of three wells. Dashed lines are the limit of quantification (LOQ; data below LOQ marked in grey). Lines represent the model prediction based on the modifications of the cell growth model described in the supporting information 4 and the parameters in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003885#pcbi-1003885-t001" target="_blank">Table 1</a> and <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003885#pcbi-1003885-t002" target="_blank">Table 2</a>. The intermediate growth phase (95%–5% proliferating cells) is indicated as grey bar.