Differential occupancy of TFs in the absence of the enhancer (MCS-R2) in mouse primary erythroblasts.

<p>The percentages of occupancy in the mutant human allele (ΔMCS-R2) was calculated by comparison with the normal human allele ( = 100%) after normalising the ChIP efficiency to an endogenous control (mouse α-globin allele). This graph shows a reduction of TFs occupancy (5′→3′) with the distance along the locus, with the lowest occupancy observed at the gene (Pr/Ex1 – Ex2 – Ex3). Figure adapted from Vernimmen et al., 2011; Genes and Development <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004668#pgen.1004668-Vernimmen5" target="_blank">[73]</a>.