(A) Experimental design for the toxicity studies in mice.

<p>Mice received an oral dose of 150 mg/kg/day of NFOH, BZL or the vehicle (9% NaCl - 5%Tween-80) once a day for 6 days per week. Mice were treated for 21 or 60 days to determine the toxicity of short-term (ST) and long-term (LT) treatment, respectively. (<b>B–D</b>) <b>Liver toxicity of anti-parasite drugs.</b> Glutamate oxaloacetate transaminase (B&D) and glutamate pyruvate transaminase (C&E) activities in mice sera (B&C) and liver homogenates (D&E) after ST and LT treatment with NFOH and BZL were determined as an indicator of liver injury. Mice treated with vehicle only were used as controls. Data in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003231#pntd-0003231-g002" target="_blank">figures 2</a>–<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003231#pntd-0003231-g007" target="_blank">7</a> are presented as mean ± S.D. (n≥9 mice per group, triplicate observations per mouse). Significance is shown as *p<0.05, **p<0.01, ***p<0.001 (vehicle control-versus-treated).