ZTF-8 is a conserved protein required for normal brood size.

<p><b>A</b>. Schematic representation of the <i>C. elegans</i> ZTF-8 protein and predicted related proteins in <i>T.porphyreolophus</i>, <i>S.Salar, N.vectensis, X.tropicalis, H.sapiens and M.musculus</i>, indicating the region deleted in the <i>tm2176</i> mutant allele. Percent similarity and identity (S and I) compared to ZTF-8 are indicated in parentheses. Protein names and/or accession numbers are indicated. <b>B</b>. Western blot analysis comparing wild type and <i>ztf-8(tm2176)</i> mutant lysates probed with N-terminal anti-ZTF-8 and anti-histone deacetylase 1 (HDA-1; loading control) antibodies. <b>C</b>. Plate phenotypes of <i>ztf-8</i> mutants. Brood size, embryonic (shown as % hatching) or larval (% adults) survivals are scored among the progeny of worms of the indicated genotypes. Error bars represent standard error of the mean. n = 24 for each genotype. Asterisks indicate statistically significant reduction compared to wild type (P<0.0001 by the two-tailed Mann-Whitney test, 95% C.I.).