Semi-quantification of wild-type PrP^Sc and MHM2^ScΔ23-88 in brains of different genotypic mice inoculated with prions.

<p>PK-treated brain homogenates from different genotypic mice inoculated with 22L (A) and RML prions (B) were analyzed for signal densities of total PK-resistant PrP and MHM2^ScΔ23-88 on Western blotting with M20 and 3F4 anti-PrP antibodies, respectively. Purified recombinant MHM2 protein was used as a reference to estimate the amounts of total PK-resistant PrP and MHM2^ScΔ23-88. Amounts of wild-type PrP^Sc were calculated by subtraction of the amounts of MHM2^ScΔ23-88 from those of total PK-resistant PrP. Data were analyzed by Student's t-test.