<p>PK-resistant PrP is detectable with M20 anti-PrP antibodies in all inoculated indicator mice, except for the mice sacrificed at 365 days after inoculation with the brain homogenate from the Tg(MHM2Δ23-88)/<i>Prnp<sup>0/0</sup></i> mouse, which was sacrificed at 591 days after inoculation with RML prions.</p
<p>(<b>A</b>) Brain homogenates from terminally ill wt and PrP(TetraH>G) line 34 mice were either le...
Ctrl; saline. dpi; days post-inoculation. *Numbers indicate the first (1) and second (2) passages. I...
a<p>Expression levels of full-length PrP<sup>C</sup> or mutant PrP are compared with those of PrP<su...
<p>Brains from terminally ill, RML-inoculated <i>Prnp<sup>0/+</sup></i> and Tg(MHM2Δ23-88)/<i>Prnp<s...
<p>PK-treated brain homogenates from different genotypic mice inoculated with 22L (A) and RML prions...
<p>Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inocu...
a<p>Expression levels of full-length PrP<sup>C</sup> in wild-type mice are estimated as 1.</p>b<p>In...
<p><b>(A)</b> Survival curves of wild-type CD-1 mice that received i.p. inoculations of rec-Prion or...
<p>All mice were inoculated with the same vCJD prion isolate. Immunoblots were analysed by enhanced ...
<p>The anti-PrP monoclonal antibodies used were, ICSM 18 (panels A, C, E, G) that recognizes both hu...
Representative blots of total brain homogenates from terminally sick WTPrPC and PrPCGPIThy-1 L150 mi...
<p>10% w/v brain homogenates (n = 3 per group) were digested with proteinase K and immunoblotted wit...
<p>A) Presence of proteinase K (PK)-resistant PrP in different brain regions of the index case. Cb, ...
<p>Immunoblots of the two PK-treated individual brains (A) and spinal cords (B) from terminally ill ...
<p>Following retrotransmission of Tg(M109)-passaged prion isolates, the biochemical characteristics ...
<p>(<b>A</b>) Brain homogenates from terminally ill wt and PrP(TetraH>G) line 34 mice were either le...
Ctrl; saline. dpi; days post-inoculation. *Numbers indicate the first (1) and second (2) passages. I...
a<p>Expression levels of full-length PrP<sup>C</sup> or mutant PrP are compared with those of PrP<su...
<p>Brains from terminally ill, RML-inoculated <i>Prnp<sup>0/+</sup></i> and Tg(MHM2Δ23-88)/<i>Prnp<s...
<p>PK-treated brain homogenates from different genotypic mice inoculated with 22L (A) and RML prions...
<p>Except for one undigested lane (−), brain samples were digested with PK (+ in panel A). All inocu...
a<p>Expression levels of full-length PrP<sup>C</sup> in wild-type mice are estimated as 1.</p>b<p>In...
<p><b>(A)</b> Survival curves of wild-type CD-1 mice that received i.p. inoculations of rec-Prion or...
<p>All mice were inoculated with the same vCJD prion isolate. Immunoblots were analysed by enhanced ...
<p>The anti-PrP monoclonal antibodies used were, ICSM 18 (panels A, C, E, G) that recognizes both hu...
Representative blots of total brain homogenates from terminally sick WTPrPC and PrPCGPIThy-1 L150 mi...
<p>10% w/v brain homogenates (n = 3 per group) were digested with proteinase K and immunoblotted wit...
<p>A) Presence of proteinase K (PK)-resistant PrP in different brain regions of the index case. Cb, ...
<p>Immunoblots of the two PK-treated individual brains (A) and spinal cords (B) from terminally ill ...
<p>Following retrotransmission of Tg(M109)-passaged prion isolates, the biochemical characteristics ...
<p>(<b>A</b>) Brain homogenates from terminally ill wt and PrP(TetraH>G) line 34 mice were either le...
Ctrl; saline. dpi; days post-inoculation. *Numbers indicate the first (1) and second (2) passages. I...
a<p>Expression levels of full-length PrP<sup>C</sup> or mutant PrP are compared with those of PrP<su...