Host CP (S100A8/A9) is elevated in <i>H. pylori</i> infected stomach tissue.

<p>A) <i>s100a8/s100a9</i> transcript abundance in RNA extracted from C57BL/6 mice infected with <i>H. pylori</i> PMSS1 or SS1 for 1, 2, or 3 months relative to uninfected animals as determined by real-time RT-PCR analysis. Points indicate mean relative units of transcript abundance +/− SEM (levels of <i>s100a8</i> in PMSS1-infected mice compared to uninfected mice; 1 mo <i>p</i> = 0.0511; 2 mo <i>p</i> = 0.0432; 3 mo <i>p</i> = 0.0127; levels of <i>s100a8</i> in SS1-infected mice compared to uninfected mice at 2 mo <i>p</i> = 0.0623 Student's <i>t</i> test). (B) Inflammation scores of <i>H. pylori</i> infected mice at 1, 2, and 3 months post infection. (C) <i>s100a8/s100a9</i> transcript abundance in RNA extracted from gastric biopsies derived from human patients, which were either <i>H. pylori</i>-positive or <i>H. pylori</i>-negative (<i>s100a8 p</i> = 0.15; <i>s100a9</i> *<i>p</i> = 0.05). Bars indicate mean relative units of transcript abundance +/− SEM. Each experimental group represents≥5 individuals (mice or human samples). D) Gastric samples derived from <i>H. pylori</i> PMSS1-infected WT mice or SS1-infected WT mice at 2 months post-infection were analyzed via immunohistochemistry using a polyclonal antibody to S100A9 (scale bars are 50 microns). E) Real-time RT-PCR was performed on gastric tissue to quantify <i>s100a8</i> and <i>s100a9</i> transcript abundance from WT (C57BL/6 mice) and IL-17RA-/- mice infected with PMSS1. Data represent relative units of transcript abundance +/− SEM in WT mice and IL-17RA-/- mice, *<i>p</i> = 0.0169 and <i>p</i> = 0.0143, respectively.