Effects of 1,25(OH)₂D₃ and 25(OH)D₃ on primary human osteoblast proliferation.

<p>Osteoblasts were cultured in the presence of 0, 1, 10 or 100 nM 1,25(OH)₂D₃ (<b>A</b>) and 0, 100, 200 or 400 nM 25(OH)D₃ (<b>B</b>) and the proliferation was quantified at day 3 and 6. Results (mean ± SEM) are expressed as treatment versus control ratios (time-point 0 was set at 1.0) using cells from 4 (A) or 7 (B) different donors. Results were analysed using Friedman test followed by Dunn’s post hoc test for each timepoint (*p<0.05, **p<0.01, ***p<0.001).