Intracellular glucose 6-phosphate and ATP levels and relative increase in serine phosphorylation levels of selected proteins of hepatocytes incubated in the presence or absence of glucose or fructose.

<p>Isolated hepatocytes were incubated for 5 min in the absence or the presence of 10 mM glucose or 10 mM fructose, as described in the Material and Methods. The table shows glucose 6-phosphate and ATP intracellular levels, as well as the relative degree of serine phosphorylation of proteins in which differences above 25% were obtained. Results are expressed in arbitrary units, transforming the intensity obtained to 100 in control points. Different superscript letters in a row indicate significant (P<0.05) differences among values from the same row. Thus, data marked with an “a” in superscript are significantly different from those marked with any other letter in the same file. Additionally, superscript asterisks indicate significant (P<0.05) differences between 10 mM Glucose values and 10 mM Glucose+75 µM Fructose ones. In both cases, differences were established following the statistical analysis described in the Material and Methods section. Acronyms for the proteins are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109726#pone-0109726-g001" target="_blank">Figure 1</a>. Results are means±S.E.M. for 5 separate experiments.</p><p>Intracellular glucose 6-phosphate and ATP levels and relative increase in serine phosphorylation levels of selected proteins of hepatocytes incubated in the presence or absence of glucose or fructose.