Hierarchical clustering of differential gene expression in the hypothalamus of non-recombinant B6.CAST-17.1-derived F₂ mice.

<p>Legend: Gene expression was measured in hypothalamus on day 2 of macronutrient selection diet using tag-based transcriptome sequencing (n = 12 individual libraries per genotype). Animals used in this experiment were either <i>cast/cast</i> (carbohydrate-preferring) or <i>b6/b6</i> (fat-preferring) within the subcongenic segment, and <i>b6/b6</i> across the rest of the genome. Genes were filtered based on ≥1.5-fold change and <i>P</i> value≤0.01 using the DESeq method. The resulting 55 differentially expressed genes are displayed on the vertical axis; individual animals are clustered by genotype on the horizontal axis. Genes whose expression changed significantly in animals with the <i>cast/cast</i> genotype (higher carbohydrate consumption), relative to <i>b6/b6</i> (higher fat consumption), cluster together as increased (red) or decreased (blue) cells, with gray representing no change. Brighter shades of red and blue reflect higher degrees of up- and down-regulation.