Mast cell degranulation is induced by mannitol in human mast cells.

<p>LAD2 mast cells were stimulated with mannitol (5%, 10%, and 17%) for 30 minutes, and β-hexosaminidase release was measured (A). CD63 staining was performed in LAD2 cells after mannitol stimulation (10%) for 30 minutes. Positive control was conducted with PMA plus ionomycin. Negative control (NC) means isotype staining (B). β-hexosaminidase content was measured in the supernatants from activated LAD2 cells, CD34+ derived human mast cells, and human lung mast cells induced by mannitol (10%) at 37°C for 30 minutes (C). Results are in triplicate and are the mean of three independent experiments expressed as mean ± standard deviation. Statistical significance *p≤0.05, **p≤0.01, ***p≤0.001) is relative to the unstimulated control cells.