The effects of <i>clathrin heavy chain</i> gene knockdown on a subsequent RNAi response as measured by <i>cathepsin L</i> knockdown in <i>M. occidentalis</i> females.

<p>(<b>A</b>) The effects of different treatment combinations on the relative mRNA levels of <i>clathrin heavy chain</i> (mean + SEM). Ingestion of <i>clathrin heavy chain</i> dsRNA resulted in a reduction of <i>clathrin heavy chain</i> mRNA levels in <i>Clathrin + Control</i> and <i>Clathrin + Cathepsin</i> mites. (<b>B</b>) The effect of different treatment combinations on the relative mRNA levels of <i>cathepsin L</i> (mean + SEM). <i>Clathrin heavy chain</i> dsRNA pretreatment reduced subsequent RNAi responses as evaluated by gene knockdown of <i>cathepsin L</i>. For <i>clathrin heavy chain</i> and <i>cathepsin L</i> mRNAs comparisons: One-way ANOVA, <i>F_3,19</i> = 470.10, <i>P</i><0.0001 and <i>F_3,19</i> = 49.16, P<0.0001, respectively, Tukey-Kramer HSD lettering for all comparisons. The mRNA levels of <i>clathrin heavy chain</i> and <i>cathepsin L</i> in <i>Control + Control</i> group are scaled to 1. Different letters denote significant differences.