Determination of α1–6-fucosyltransferase activity.
- Sylvie Juliant (647588)
- Anne Harduin-Lepers (647589)
- François Monjaret (647590)
- Béatrice Catieau (647591)
- Marie-Luce Violet (647592)
- Pierre Cérutti (647593)
- Annick Ozil (647594)
- Martine Duonor-Cérutti (647595)
Publication date
October 2014
DOIAbstract
<p>Recombinant FLAG-tagged <i>Sf</i>9 FUT8 was produced using the baculovirus-<i>Sf</i>9 cell system and purified using anti-FLAG M2 affinity gel. The fucosyltransferase activity of the recombinant protein was tested with GDP-[<sup>14</sup>C]-L-fucose, as substrate, after two days (2D) and three days (3D) of production. The transfer of [<sup>14</sup>C]-fucose is expressed in cpm.</p><p>Determination of α1–6-fucosyltransferase activity.</p
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