Quantitative proteomic analysis of mouse brush samples.

<p>(A) LC-MS/MS analysis of mouse proteins in vaginal brush samples that are affected by treatment with N9 (open, black circles) or BZK (closed squares). Shown are proteins that are increased compared to vehicle controls. The following proteins are depicted in the graph: fibrinogen alpha polypeptide isoform 2 (FGA), plasminogen (PLG), apolipoprotein A-1 (Apo A-1), and apolipoprotein C-1 (Apo C-1). Each data point represents the average of three replicates per individual experimental study. Only proteins shown to change in four studies are represented. (B) ELISA analysis of mouse proteins in vaginal brush samples that are increased in response to treatment with 8% N9 or 2% BZK. Statistical analysis was performed using a two-tailed Student's T-test of N9 and BZK samples compared to vehicle controls (**** = p≤0.0001, ** = p≤0.01). (C) ELISA analysis of mouse proteins in vaginal brush samples that are increased in response to treatment with vehicle, 1% N9, 4% N9, or 8% N9. Statistical analysis was performed using a two-tailed student's t-test of N9 and BZK samples compared to vehicle controls (**** = p≤0.0001, *** = p≤0.001, ** = p≤0.01, * = p≤0.05, NS = not significant).