Effect of NADPH oxidase inhibitors (DPI, PY, and IMZ) on endogenous O₂^{• −} (A), endogenous total ROS (B–C), lipid peroxidation (D), the loss of plasma membrane integrity (E), and the growth of roots under Se(IV) stress.

<p>The roots of seedlings were exposed to Se(IV) (0.06 mM), Se(IV) (0.06 mM)+DPI (10 µM), Se(IV) (0.06 mM)+PY (5 mM), and Se(IV) (0.06 mM)+IMZ (0.5 mM) for 48 h. Afterwards, the roots were stained with NBT (A), DCFH-DA (B), Schiff's reagent (D), and Evan blue (E), respectively. The density of DCF fluorescence were estimated (C). The roots of seedlings were exposed to the above treatment solutions for 72 h. Afterwards, the root length were measured (F). <i>Asterisk</i> indicates that mean values of three replicates are significantly different between the different treatments (<i>P</i><0.05).