Tat does not affect other CD4 T-cell surface proteins.

<p>Purified CD4 T-cells from healthy HIV-negative volunteers (n = 4) were incubated in medium alone or with Tat protein (10 µg/ml) for up to 48 hours and then analyzed by flow cytometry. (A) Changes in positive staining for the indicated surface proteins relative to medium controls (p>0.09 for all proteins other than CD127 where p<0.04). (B) Change in CD132+ CD4 T-cells relative to medium controls (p>0.12 at 48 hours). Graph represents mean values ± SEM. Representative flow histograms show CD4 T-cells from one individual incubated in media (grey fill) or media plus Tat (black line) at 48 hours.