Flow cytometry analysis of AT-resident leukocyte populations.

<p>(A) H&E staining of isolated leukocyte populations from the rainbow trout AT. Scale bar, 5 µm. (B) Flow cytometry analysis of leukocytes isolated from the AT stained with MAbs against trout IgM, IgT, IgD, CD8 and MHC-II. Dot plots show a FSC/SSC profile of isolated AT-resident leukocytes including the leukocyte gate and corresponding antibody fluorescence intensity versus FSC of gated leukocytes. (C) Mean percentage of IgM⁺, IgT⁺, IgD⁺, CD8α⁺ and MHC-II⁺ leukocytes in the gated population (n = 8).