Phytochrome A is stabilized by white light of very low R:FR.

<p>(<b>a</b>) phyA levels were assayed in extracts from Col-0 seedlings grown in darkness for 5 days and then either transferred to W or W+FR for 4 hours. Circles indicate the harvest time of the plant material. (<b>b</b>) Representative steady-state levels of phyA (upper panel) and tubulin (TUB, lower panel) in extracts from seedlings grown as indicated in <b>a</b>,. Bands were detected by immunoblot using the phyA-specific mAb 073D or a TUB-specific mAb. TUB was used as a loading control. (<b>c</b>) Relative levels of phyA normalized to TUB in seedlings differentially grown for 4 h under W or W+FR, as indicated in <b>a</b>; n = 3 independent biological replicas; the P-value between the W and W+FR treated samples was 0.053.