<p>H295R cells were treated with 8-Br-cAMP or forskolin for 48 h under growth conditions, and fixed with 4% paraformaldehyde. (A) Immunostaining for CYP17A1. Red staining shows the anti-CYP17A1 antibody and blue staining shows DAPI (cell nuclei). (B) Immunostaining for CYP21A2. Red staining shows the anti-CYP21A2 antibody and blue staining shows DAPI (cell nuclei). Scale bars represent 100 µm.</p
<p>Cultured human scleral fibroblasts (HSFs) were treated with 10 µM forskolin for 30 min. Forskolin...
<p>(A) Representative images showing changes in fluorescence intensity (FI) by 50 µM forskolin appli...
<p>(a) Representative DNA histograms of cells following treatment with indicated concentrations of C...
<p>- <b>and 3D-cultured cells.</b> (A) cAMP levels are higher in 3D-cultured cells compared to 2D-cu...
<p>H295R cells were treated and fixed as in <a href="http://www.plosone.org/article/info:doi/10.1371...
<p>At 1 h before GIP stimulation, GIPR-transfected H295R cells were treated with or without ACTH (7–...
<p>(A) Relative mRNA expression of the indicated genes was analyzed by qRT-PCR. RNA was extracted fr...
<p>Cells were analyzed for changes in the phosphorylation status of Smad3 (A) and CREB (B) as well a...
Many different types of cells exhibit a supersensitivity of aden-ylate cyclase after chronic treatme...
<p>(<b>A</b>) PC12 cells treated with forskolin (10 μM) or DMSO as control for 2 h to perform qPCR. ...
<p>AZF cells were incubated either without (control), or with 8CPT-2′-OMe-cAMP, 8CPT-2′-OMe-cAMP plu...
<p>(A and B) The time course of the <i>I</i><sub>K</sub> changes induced by the intracellular applic...
<p>Cells were pre-incubated with 100 μM IBMX for 20 min, followed by incubation with 10 μM forskolin...
<p>The potency (IC<sub>50</sub>) and efficacy (E<sub>MAX</sub>) for modulation of forskolin-stimulat...
<p>A549 cells were untreated or treated with 500 nM of 1, 9, 12, 16, 32, 33, and 35 for 24 h. Follow...
<p>Cultured human scleral fibroblasts (HSFs) were treated with 10 µM forskolin for 30 min. Forskolin...
<p>(A) Representative images showing changes in fluorescence intensity (FI) by 50 µM forskolin appli...
<p>(a) Representative DNA histograms of cells following treatment with indicated concentrations of C...
<p>- <b>and 3D-cultured cells.</b> (A) cAMP levels are higher in 3D-cultured cells compared to 2D-cu...
<p>H295R cells were treated and fixed as in <a href="http://www.plosone.org/article/info:doi/10.1371...
<p>At 1 h before GIP stimulation, GIPR-transfected H295R cells were treated with or without ACTH (7–...
<p>(A) Relative mRNA expression of the indicated genes was analyzed by qRT-PCR. RNA was extracted fr...
<p>Cells were analyzed for changes in the phosphorylation status of Smad3 (A) and CREB (B) as well a...
Many different types of cells exhibit a supersensitivity of aden-ylate cyclase after chronic treatme...
<p>(<b>A</b>) PC12 cells treated with forskolin (10 μM) or DMSO as control for 2 h to perform qPCR. ...
<p>AZF cells were incubated either without (control), or with 8CPT-2′-OMe-cAMP, 8CPT-2′-OMe-cAMP plu...
<p>(A and B) The time course of the <i>I</i><sub>K</sub> changes induced by the intracellular applic...
<p>Cells were pre-incubated with 100 μM IBMX for 20 min, followed by incubation with 10 μM forskolin...
<p>The potency (IC<sub>50</sub>) and efficacy (E<sub>MAX</sub>) for modulation of forskolin-stimulat...
<p>A549 cells were untreated or treated with 500 nM of 1, 9, 12, 16, 32, 33, and 35 for 24 h. Follow...
<p>Cultured human scleral fibroblasts (HSFs) were treated with 10 µM forskolin for 30 min. Forskolin...
<p>(A) Representative images showing changes in fluorescence intensity (FI) by 50 µM forskolin appli...
<p>(a) Representative DNA histograms of cells following treatment with indicated concentrations of C...