DEX responsiveness is abolished when the C/EBPβ site is mutated.

<p>The rat <i>Cbg</i>295Luc promoter reporter construct with various mutations at regulatory sites C/EBPβ (P5), HNF3 (P4), and DBP (P3) were transiently transfected into COS-7 cells. Transfected cells were treated with 10 µM DEX or vehicle control (0.1% EtOH) for 24 hrs. Luciferase activity was measured and normalized to protein concentrations. Results were compared relative to the corresponding vehicle control (0.1% EtOH). For statistical analysis two-way ANOVA was done with Bonferroni’s multiple comparison as post-test to compare DEX-induced treatments with each corresponding control (0.1% EtOH) (*: p<0.05; **: p<0.01; ***: p<0.001).