Viral induced deletion of XBP-1 impairs viral DNA replication and gene expression in vivo.

<p>XBP-1^WT/WT and XBP-1^f/f mice infected with 10∧5 PFU of MCMV-CRE. Mice were sacrificed 4 dpi and livers were extracted. (A) Viral genome copy number in mg tissue was determined using quantitative RT-PCR analysis of viral M55(gB) and the host gene pThrp (n = 3 (XBP-1^f/f), n = 4 (XBP-1^WT/WT)). The expression of representative viral RNAs (IE1, E1 and M55(gB)) in liver was measured by RT-PCR analysis and calculated relative to WT animals. Results are shown as mean ±SEM. * P<0.05, ** P<0.01.